Effects of various handling and storage conditions on stability of Treponema pallidum DNA in cerebrospinal fluid.
نویسندگان
چکیده
Treponema pallidum DNA from even small numbers of organisms was detectable in cerebrospinal fluid (CSF) stored at room temperature or at 4 degrees C for several hours and in CSF subjected to three freeze-thaw cycles. These results suggest that negative PCR results for T. pallidum from patients diagnosed with T. pallidum invasion of the central nervous system are probably not due to the loss of target DNA prior to testing.
منابع مشابه
Detection of Treponema pallidum subsp. pallidum from skin lesions, serum, and cerebrospinal fluid in an infant with congenital syphilis after clindamycin treatment of the mother during pregnancy.
We report here a case of congenital syphilis in a newborn after clindamycin treatment in pregnancy. Using PCR detection of tmpC (TP0319) and DNA sequencing of the genes TP0136 and TP0548, DNA sequences identical to Treponema pallidum subsp. pallidum strain SS14 were detected in the infant's skin lesions, serum, and cerebrospinal fluid.
متن کاملInfant with Congenital Syphilis after Clindamycin Treatment in Pregnancy
26 We report a case of congenital syphilis in a newborn after clindamycin treatment in pregnancy. 27 Using PCR detection of tmpC (TP0319) and DNA sequencing of genes TP0136 and TP0548, DNA 28 sequences identical to Treponema pallidum subsp. pallidum strain SS14 were detected in the infant’s 29 skin lesions, serum, and cerebrospinal fluid. 30 31
متن کاملConcerning Agglutinins for Treponema Pallidum
1. There is no demonstrable amount of agglutinin for Treponema pallidum (Noguchi) in normal human and normal rabbit serum in dilutions as low as 1:20. 2. Agglutinins for Treponema pallidum are readily produced in young rabbits by the administration of pure cultures of living spirochetes. 3. There is no appreciable amount of agglutinin for Treponema pallidum culture used in the sera of secondary...
متن کاملCerebrospinal fluid immunoglobulins in neurosyphilis.
Using the fluorescent treponemal antibody-absorption (FTA-ABS) test and the solid phase haemadsorption assay (SPHA) Treponema pallidum-specific IgA was found in the cerebrospinal fluid (CSF) of patients with neurosyphilis but not in those with late latent syphilis. The presence of T pallidum-specific IgA in the CSF may inhibit the antitreponemal activity of IgG and thus play some part in the pa...
متن کاملMolecular Typing of Treponema pallidum: A Systematic Review and Meta-Analysis
BACKGROUND Syphilis is resurgent in many regions of the world. Molecular typing is a robust tool for investigating strain diversity and epidemiology. This study aimed to review original research on molecular typing of Treponema pallidum (T. pallidum) with three objectives: (1) to determine specimen types most suitable for molecular typing; (2) to determine T. pallidum subtype distribution acros...
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 36 7 شماره
صفحات -
تاریخ انتشار 1998